FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21490223
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA32000.013.6550544
CGACCTA17550.012.7196844
CGTATCG28700.012.5772953
CCCGTAT38250.010.9374041
CGGGGAT74250.010.24441
CGACCGT15700.09.9925721
CGCGAAC10203.6379788E-119.7877331
CGCCAAT40850.09.7757531
TACGCAC19850.09.5703499
CGAAGAT87200.09.5407951
CCGTATC41800.09.4352952
CGGGATT32950.09.2339511
CGCAAAT28450.09.0234891
CGCGTAA4250.0073894998.9487851
ATCGAAG65450.08.8527566
TATCGAA48500.08.81325155
CGCGGAT24900.08.7825681
CGGGGTT72750.08.7565861
CCGGAAT50600.08.7376661
CTCGGAT68700.08.7192031
Produced by FastQC (version 0.11.5)