FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20678414
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG22150.013.9320143
GTATCGA29900.012.8616454
CGACCTA17800.011.4691484
CGCGGGT30150.011.3395861
CCCGTAT35650.011.0553021
CCGCAAT37300.010.9481731
CGCGTAT3953.3706566E-410.8192581
CGACCGT13900.010.5900731
CGGGGAT54550.010.0975321
CCGGGAT53950.09.9457841
CGGGGTT67950.09.5038921
CGCAAAT30300.09.24616051
CGGGAAT58050.09.0797271
CGAAGAT87300.08.9747361
CGGACAT52650.08.9287131
CCGGAAT52450.08.8722261
GATCGGA66000.08.85778894-95
CCGTATC42150.08.7867242
CGGCAAT35900.08.7297451
CGCCAAC53800.08.6495961
Produced by FastQC (version 0.11.5)