FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18597771
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA26950.012.5163284
CGTATCG21700.012.4793643
CGACCTA14400.011.877294
CGGTAAT21100.011.032311
CCGCAAT42750.011.00151
CGGCGAA17500.010.3156921
CCGGGAT45650.010.0944771
CGACGCT22650.010.0675981
CCGGTAA21650.09.8743261
CGAAGAT73050.09.6898911
GTTAACG11550.09.66415716-17
CAATCGG40250.09.560854
CGGCGAT46500.09.5012961
GCGAACG12151.8189894E-129.3845263
TCAATCG35200.09.3128753
CCCGTAT41400.09.2947461
CGCGAAT10751.07320375E-109.2803351
CTCGGAT69300.08.9117491
TAAGGCG37050.08.8473378
CGCGGAT19900.08.8328631
Produced by FastQC (version 0.11.5)