FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19661283
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA17550.014.0703894
CGCGAAT11300.013.0404611
CGCGTAT4609.465071E-611.3669661
CCGCAAT37750.011.3327481
TACGCAC18450.011.3256099
CGCGGGT26800.010.9968071
CGTATCG20300.010.9947693
CCCGTAT31100.010.0877261
CGAAGAT86000.09.94910051
GTATCGA28200.09.9353414
CGACTAT11600.09.8347431
CCGGGAT49250.09.748211
CGCCAAT41600.09.598331
CGACGAT22150.09.2279331
CGCAAAT26450.09.165461
CGCCAAC50000.09.0315711
GTCCGAT15350.08.6708041
CGGCAAT33000.08.6426521
CGCTAAT9357.749986E-88.6426521
GATCGGA62250.08.62104394-95
Produced by FastQC (version 0.11.5)