FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19923468
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT13350.017.4366321
CGACCTA17950.016.6782514
TACGCAC23800.012.7743839
CGTATCG22300.010.4416713
GTATCGA24450.010.3008124
CCCGTAT31400.09.9853231
CTCGGAT63400.09.8908241
CGCAGAT48200.09.8560021
CGCCAAT39600.09.8370861
CGCGAAC9701.4915713E-109.7950371
CCGGCAT54600.09.6577991
ATCGAAG52950.09.332556
CGAAGAT67250.09.2539431
CGCGGAT21650.09.2159311
CGGCAAT35200.09.0423211
CGGACAT46850.09.0246061
CTGGAAT196150.09.0095371
GACGACC45850.08.9110252
CGAGAAT42150.08.903841
CTACGCA40600.08.8940328
Produced by FastQC (version 0.11.5)