FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19398434
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG24150.019.6673893
CGACCGT11900.017.5783621
CCCGTAT34450.016.1461621
CGACCTA14950.015.5673744
GTATCGA30100.014.9905514
CCGTATC34800.014.6105142
TACCGTA19100.011.1893519
ATCGAAG56500.010.9274976
CGCGTAT10200.010.7201361
CGCGAAT9951.8189894E-1210.5116831
TATCGAA46850.010.1373935
CTCGGAT57600.09.9870121
CCGGGAT45750.09.9759161
TACGCAC20050.09.948579
CGCGATT7301.768949E-79.7687971
TATCGCG5154.5474735E-119.68321522-23
CTCGGGT55400.09.4396431
CTCGGTT35050.09.3590921
CGGGTAT16850.09.3107931
CCCGGTT36500.08.9872931
Produced by FastQC (version 0.11.5)