FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23392134
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA19000.015.7483334
CGTATCG25800.014.3589463
GTATCGA31200.012.6348814
CCCGTAT35900.012.4902591
CGACCGT16350.012.2537661
TACGCAC26500.011.829629
CCGTATC40550.010.1900452
CCGGGAT65900.010.1339951
CGCGGGT41950.09.892941
CTCGGAT71750.09.7731271
CGCGATT8056.858863E-89.4811741
TATCGCG6300.09.42368720-21
CGGGAAT57400.09.2246341
CGCCAAT47650.09.2100711
ATCGAAG63100.09.1828636
CCGGCAT59700.09.1089551
CCGGAAT56800.09.0701291
CGCGAAC12101.0913936E-119.0673531
CTTGGAT178550.08.9232841
CGGACAT55150.08.8225211
Produced by FastQC (version 0.11.5)