FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23392134
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA20800.021.2416934
TACGCAC27200.015.5428389
CGTATCG26650.013.3700683
GTATCGA29150.013.03830054
CGACCGT17300.012.6317271
CGCGTAA4951.710283E-611.5166721
CCCGTAT37250.010.8403581
CGCGGAT23250.010.2164041
CGAAGAT82900.010.1430781
CGCGAAT12400.09.9609931
CTACGCA47850.09.63017758
GACGACC53850.09.6163522
CTCGGAT72400.09.4487621
CTCGTAT28000.09.331591
CCGGGAT65150.09.1877061
ATCGAAG63500.09.1271716
GACCTAC71450.09.1089525
ACGACCT53450.09.0661323
CGCGTAT4850.0022033698.8155981
GATCGGA86600.08.7891294-95
Produced by FastQC (version 0.11.5)