FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21977661
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT15950.015.4978111
CGCGGGT39950.014.7547741
CCGGGAT65650.011.29581451
CGCGAAT11900.010.7856041
CGAAGAT74950.09.6405011
CGAGAAT47100.09.4871251
CGCGATT8251.0557051E-79.2192111
CGGGAAT56350.09.195181
CGTATCG20750.09.1544153
GTCGATT28800.09.0781611
CCGAGAT90800.08.7429561
GCGGGTC63500.08.6751892
CCGACAT52700.08.65940951
CGCAAAT31450.08.4643771
ACGGAAC39350.08.3909634-35
CGGGGTT69900.08.36481
CCGGCAT57200.08.3105861
GATCGGA73000.08.2947494-95
CGGGGAT69350.08.2255021
CGCAGAT53750.08.2249291
Produced by FastQC (version 0.11.5)