FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15080928
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG19550.015.3053593
GTATCGA26200.013.0521174
CGACCGT12500.012.2122171
CCCGTAT29800.011.8459531
GACGCTA21400.010.875074
CCGTATC31350.010.301992
CGCGAAT10750.010.2064311
TAGGTCG11250.010.1331497
CGCGGAT17900.09.8605981
CGACCTA10355.0931703E-119.6367074
GTTCGAT17950.09.567371
CGCGTAT4000.00436232569.5407951
CCGCAAT22150.09.4761851
TATCGAA38950.09.3892965
ACGCTAC24350.09.3625045
CGCATAC9205.686161E-99.3333861
CTCGGGT44750.09.2742921
ATCGAAG49250.09.2579486
CCGGGAT40350.09.2215861
CGCGCAT8451.4446778E-79.0327051
Produced by FastQC (version 0.11.5)