FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15080928
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG21350.018.2472083
GTATCGA26900.014.8356794
CGACCGT12450.014.1181181
CCCGTAT31150.013.5730171
CCGTATC35350.012.2301672
CGCGAAT10350.011.0157821
CGCCAAC40850.010.6989261
GACGCTA18650.010.44444754
TATCGAA41850.010.4438665
CGCCAAT26200.010.153861
CGGACAT33250.010.001171
CTCGGGT43900.09.8473941
ATCGAAG53250.09.8139086
ACGCTAC21900.09.7619675
CGAAGAT54900.09.5184181
CGCGATT5506.8365516E-59.5011111
CGGAAAT49000.09.4041621
CTCGAAT36300.09.291721
AATCGGC15950.09.2335765
GTCGATT11857.2759576E-129.2204881
Produced by FastQC (version 0.11.5)