FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18409694
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG21400.016.6547833
CCCGTAT29850.014.483121
GTATCGA27200.013.1033954
CGACCTA16000.012.17742354
CGCGTAT8551.8189894E-1211.6685871
CGCGGAT21600.011.4370671
CCGCAAT26400.010.9771851
CGGGGTT59800.010.8838981
TCGGGTC38450.010.7501082
CGCGAAT7703.2941898E-910.4887391
CTCGGTT30850.010.3177431
CTCGGGT50250.010.2106351
ATCGAAG53200.010.0930046
CGACCGT12650.09.7644521
CCGTATC39100.09.7208242
CCGTAAT19800.09.5975391
TACGCAC20400.09.3150049
CGGGAAT42150.09.2423281
CCCGGTT34600.09.1994911
CGCGGTT19550.08.9912491
Produced by FastQC (version 0.11.5)