FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18577815
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG22600.018.2835033
GTATCGA29300.015.2373284
CGCGTAT8200.015.1251561
CCCGTAT30300.015.1136381
CGACCTA14850.014.3924474
CCGTATC32750.014.2122672
CGCGAAT10250.012.1001251
CGACCGT11600.011.9256041
TATCGAA41700.011.3897075
CGCGTAA2950.005026153311.3192181
TACGCAC17250.010.7385669
CGGGGTT56650.010.44148351
ATCGAAG54000.010.2905996
CTCGGGT54250.09.8482411
CGCGATT6908.2255065E-79.6787511
CGGGGAT56350.09.565881
TCGGGTC41700.09.4534562
CTCGGTT32350.09.43726351
CCGGCAT48850.09.2768271
GACGCTA23150.09.2323044
Produced by FastQC (version 0.11.5)