FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19137334
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA26950.015.3265874
CGACCTA16600.014.8723724
CGTATCG24400.014.2040553
CGCAAAT22400.012.2954181
TACGCAC19150.012.1449399
GACGCTA22200.011.5485054
CGGTAAT23750.011.196641
CGCCAAT33600.011.1648041
CGCGCAT7753.6925485E-910.4162281
CTCGGTT28400.010.03221
TCGACTC26650.09.9778272
CCGGGAT45650.09.882031
CTCGGAT67850.09.8680781
CCCGTAT43450.09.508081
CCGGCAT48750.09.4484471
CGACCGT12101.8189894E-129.4186611
CGCGAAT11102.0008883E-119.4115911
CGAAGAT75050.09.3642791
CCGGTAA21550.09.2547631
ACGCTAC29450.09.1891335
Produced by FastQC (version 0.11.5)