FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22969326
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT42700.015.6802681
CGTATCG22700.011.7127063
CCGGGAT67000.010.5602371
CGGGAAT54900.010.0333941
GTATCGA26750.09.9394884
CCCGTAT33250.09.8541611
CGGGGAT74300.09.4587791
CGGGTCT98650.09.3368363
CTCGGAT68000.09.0083041
CCGGGTT78650.08.935631
CCGGAAT57500.08.919041
CGGACAT51050.08.8366991
GCGGGTC70500.08.7558542
CGCGAAC13651.8189894E-128.6970021
CTCGGGT64550.08.6805651
ACGGAAC44900.08.67399434-35
CGACCGT16900.08.4294021
CGCCATT62400.08.3708641
CGGCAAC39800.08.3517441
CGCGAAT13107.094059E-118.3371721
Produced by FastQC (version 0.11.5)