FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19217096
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT13150.015.1816741
CGCGGGT30650.014.577851
CGCGTAT3558.359411E-613.3896051
CGTATCG24500.012.2111253
CGCGATT6452.2973836E-911.7911561
GTATCGA30350.010.7961624
CGCAAAT25250.010.5419941
CGGGGTT60300.010.3264281
CCCGTAT35300.010.2337541
ACGCTAC27100.09.8133415
CCGGGAT50450.09.79869651
GACGCTA25250.09.7795964
CTTGGAT146800.09.7462281
CGGGTCT71050.09.691373
CGCGACT11403.45608E-119.1730551
CCGAAAT37400.09.1507571
CTCGGGT52500.09.1444631
CCGGGTT56400.09.1020821
CGAAGAT67050.09.0741781
CGGGAAT55450.08.9151351
Produced by FastQC (version 0.11.5)