FastQCFastQC Report
Fri 13 May 2022
EGAF00005307593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700181277
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG25381321536.24964324774426TruSeq Adapter, Index 8 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT122244291.7458948705936335TruSeq Adapter, Index 8 (97% over 36bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG16780090.2396535090440586TruSeq Adapter, Index 3 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCACGTATG16465750.2351641002248622TruSeq Adapter, Index 8 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTATG15946890.22775373355206127TruSeq Adapter, Index 8 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCCCGTATG11317750.16164028333479705TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)