FastQCFastQC Report
Mon 30 May 2022
EGAF00005307595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419872790
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG5774019413.751830405585464TruSeq Adapter, Index 3 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCCCGTATG10771580.2565438927347495TruSeq Adapter, Index 3 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTATG4900580.11671582719137384TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)