FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005307700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46779
Sequences flagged as poor quality0
Sequence length246-251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9852.105645695718164No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9832.1013702729857417No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7761.6588640201799953No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA7291.5583915859680626No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7011.4985356677141453No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6601.4108895016994805No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5291.1308493127257957No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5231.1180230445285277No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA4861.0389277239787083No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4791.023963744415229No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4440.9491438465978324No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4420.9448684238654097No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4410.9427307124991983No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4390.9384552897667757No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3950.844395989653477No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3880.8294320100899977No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3840.8208811646251523No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3720.7952286282306162No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA3520.7524744009063896No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA3510.7503366895401783No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA3410.7289595758780649No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3230.690480771286261No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3200.6840676371876269No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3200.6840676371876269No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3170.677654503088993No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3080.658415100793091No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3020.6455888325958229No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3000.6413134098634002No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2930.6263494302999209No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2910.6220740075674982No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2790.5964214711729622No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2760.5900083370743282No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2670.5707689347784263No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2650.5664935120460035No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2570.5493918211163129No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2460.5258769960879882No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2060.4403685414395348No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2010.42967998460847817No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2000.42754227324226685No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1980.42326685050984414No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1960.41899142777742143No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1840.39333889138288547No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1810.3869257572842515No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1800.3847880459180401No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1790.38265033455182884No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1750.3740994890869835No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1680.35913550952350415No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1660.35486008679108144No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1480.3163812821992774No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1400.2992795912695868No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1380.2950041685371641No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1370.2928664571709528No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1340.2864533230723188No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1340.2864533230723188No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1300.27790247760747344No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1280.27362705487505073No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1180.2522499412129374No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG1170.2501122298467261No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1110.2372859616494581No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1100.23514825028324676No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1070.22873511618461276No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1040.22232198208597875No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1030.2201842707197674No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1020.2180465593535561No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1010.21590884798734475No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG990.21163342525492207No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG970.20735800252249942No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG930.19880715705765406No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG920.19666944569144273No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG890.19025631159280873No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG880.1881186002265974No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA880.1881186002265974No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG870.18598088886038608No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG870.18598088886038608No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG850.1817054661279634No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG850.1817054661279634No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA840.17956775476175207No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG830.17743004339554072No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG830.17743004339554072No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG800.17101690929690672No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG760.1624660638320614No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG760.1624660638320614No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG750.16032835246585006No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG740.1581906410996387No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG730.15605292973342738No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG730.15605292973342738No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG720.15391521836721606No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG720.15391521836721606No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG720.15391521836721606No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG680.14536437290237073No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG660.14108895016994805No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG650.13895123880373672No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG640.13681352743752537No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG630.13467581607131404No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG620.13253810470510272No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA620.13253810470510272No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG610.1304003933388914No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG590.1261249706064687No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG560.11971183650783472No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG560.11971183650783472No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG560.11971183650783472No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCACTTCAG550.11757412514162338No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG540.11543641377541203No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG530.1132987024092007No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG520.11116099104298938No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA510.10902327967677805No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA510.10902327967677805No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG500.10688556831056671No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG500.10688556831056671No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG490.10474785694435536No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG480.10261014557814403No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG470.1004724342119327No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG470.1004724342119327No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)