FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93676
Sequences flagged as poor quality0
Sequence length246-251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG26272.8043468978180113No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG18061.9279217729194245No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15741.6802596182586789No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG12741.360006832059439No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG12731.358939322772108No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG11361.2126905504077885No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG11281.2041504761091422No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG10941.167855160339895No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10321.1016695845253852No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG9571.0216063879755755No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8850.9447457192877577No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8550.9127204406678339No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG7840.8369272812673471No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7610.8123745676587387No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7430.7931594004867842No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7390.7888893633374611No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7360.7856868354754687No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7050.7525940475682139No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG6830.7291088432469363No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6260.6682608138690806No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG6110.6522481745591187No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG5990.6394380631111491No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5970.6373030445364875No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5500.5871301080319399No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5260.5615098851360006No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5040.5380246808147231No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4890.5220120415047611No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG4680.49959434647081424No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG4660.49745932789615266No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4630.4942568000341603No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4590.4899867628848371No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4480.47824416072419834No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG4420.4718391050002135No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4320.4611640121269055No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG4250.45369144711558995No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4240.45262393782825905No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4180.4462188821042743No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4160.4440838635296127No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4130.44088133566762033No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4050.43234126136897394No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3850.4109910756223579No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3810.40672103847303476No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG3760.4013834920363807No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3750.4003159827490499No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3440.3672231948417951No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3390.3618856484051411No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3370.3597506298304795No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG3260.34800802766984074No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3260.34800802766984074No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3150.3362654255092019No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3120.33306289764720953No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG3040.32452282334856314No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3040.32452282334856314No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG2940.31384773047525516No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2860.3053076561766087No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG2850.3042401468892779No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG2840.3031726376019471No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2740.29249754472863915No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG2680.28609248900465434No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2670.28502497971732355No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG2650.28288996114266196No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2600.27755241470600794No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2490.2658098125453692No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2450.26153977539604595No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2440.26047226610871516No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2400.25620222895939193No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2380.25406721038473035No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2340.24979717323540712No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2310.24659464537341474No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2280.24339211751142234No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2270.24232460822409155No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2260.24125709893676076No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2240.23912208036209917No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG2220.23698706178743753No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2210.23591955250010674No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2200.23485204321277595No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA2180.23271702463811436No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2140.22844698748879116No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG2130.22737947820146037No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2010.21456936675349078No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG1980.21136683889149835No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1970.21029932960416756No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1950.20816431102950597No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1940.20709680174217515No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1740.18574661599555917No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1690.18040906955890515No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1660.17720654169691277No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA1640.17507152312225116No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1620.17293650454758958No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCAG1550.16546393953627395No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1490.15905888381228916No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1470.15692386523762758No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1460.15585635595029676No Hit
GACTACTGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG1450.15478884666296597No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1440.15372133737563518No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1430.15265382808830436No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1410.15051880951364277No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCAG1320.14091122592766556No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1320.14091122592766556No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1320.14091122592766556No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1280.13664118877834236No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1250.13343866091634998No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA1230.13130364234168837No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1220.13023613305435758No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1210.12916862376702679No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG1180.12596609590503438No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1150.12276356804304198No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1140.12169605875571117No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCAG1100.11742602160638797No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA1100.11742602160638797No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA1080.11529100303172637No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1080.11529100303172637No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1020.10888594730774158No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1010.10781843802041079No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCAG990.10568341944574917No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA990.10568341944574917No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA980.10461591015841838No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG970.10354840087108758No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG960.10248089158375678No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG940.10034587300909517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA309.094947E-12266.67383245
ATCAGGA201.0178883E-7266.67383245
ATTCGGA800.0266.67383245
ATACGGA151.0798349E-5266.67383245
ATTCTGA100.0011434611266.67383245
ATCTGGA151.0798349E-5266.67383245
TTCCGGA13200.0264.6536245
ATCCGGA13400.0262.6936245
ATTCCGA9100.0252.02142245
AACTACT100.0014736671245.020841
GACGACT100.0014736671245.020841
GACTCCT100.0014736671245.020841
GACTACT36600.0245.020831
GAATACA151.5144149E-5245.020811
GATTACT151.5144149E-5245.020811
GACTATT151.5144149E-5245.020811
GACTAAA151.5144149E-5245.020811
TGGGGTA18650.0244.890037
AAGGGTA9800.0244.890037
GGGGCAT151.5176482E-5244.890018
Produced by FastQC (version 0.11.5)