FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66137
Sequences flagged as poor quality0
Sequence length35-251
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT18882.854680436064533No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT18792.8410723195790557No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT18322.7700077112660084No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT17142.5915901840119755No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC16302.4645810968141886No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC13762.080529809335168No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11341.7146226771701165No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT11131.6828704053706698No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10951.6556541723997158No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA10891.646582094742731No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA10681.6148298229432843No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10671.6133178100004537No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT9701.4666525545458668No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC8991.3592996356048808No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8921.3487155450050652No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC8621.3033551567201416No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA7441.1249376294661082No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA7361.1128415259234619No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC7341.1098175000378003No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT7091.0720171764670305No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6811.0296808140677685No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6701.0130486716966296No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6651.0054886069824758No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6540.988856464611337No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC6450.97524834812586No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA6420.9707123092973675No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC6410.9692002963545369No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6350.9601282186975522No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC6240.9434960763264133No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5840.8830155586131818No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA5740.8678954291848738No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC5410.8179990020714577No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5340.8074149114716421No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5300.801366859700319No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA4980.7529824455297337No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4660.7045980313591484No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4510.6819178372166865No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA4070.6153892677321318No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4010.6063171900751471No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA3980.6017811512466548No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3950.5972451124181622No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3850.5821249829898544No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA3830.5791009571041927No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3810.5760769312185312No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA3740.5654928406187156No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3730.5639808276758849No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3520.5322285558764384No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA3280.4959402452484993No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3240.48989219347717616No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3070.4641879734490527No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3000.4536038828492372No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3000.4536038828492372No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2950.44604381813508326No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2900.4384837534209293No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2840.42941167576394457No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA2590.3916113521931748No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2550.3855633004218516No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2550.3855633004218516No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2510.37951524865052844No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA2380.35985908039372816No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2360.35683505450806663No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2290.34625096390825105No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2130.3220587568229584No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA2120.3205467438801276No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2120.3205467438801276No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC2040.3084506403374813No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC2010.3039146015089889No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1910.288794472080681No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1820.2751863555952039No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1790.27065031676671153No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1770.26762629088104994No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1680.2540181743955728No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1620.24494609673858808No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA1620.24494609673858808No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1610.2434340837957573No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1600.2419220708529265No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1590.2404100579100957No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1530.23133798025311098No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1530.23133798025311098No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA1440.21772986376763387No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1430.21621785082480308No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1430.21621785082480308No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1410.2131938249391415No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1310.19807369551083356No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1280.19353765668234119No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1240.18748960491101804No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1180.17841752725403331No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1160.17539350136837173No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1150.17388148842554094No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1130.17085746253987932No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA1130.17085746253987932No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1100.16632142371138697No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1100.16632142371138697No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1090.16480941076855618No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1090.16480941076855618No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1050.158761358997233No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1040.15724934605440222No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1030.15573733311157145No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1030.15573733311157145No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT1020.15422532016874066No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1000.15120129428307907No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT990.14968928134024825No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCACAATGGAGGAAACTCTGAT990.14968928134024825No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC990.14968928134024825No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC980.14817726839741746No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA970.1466652554545867No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA960.1451532425117559No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT950.1436412295689251No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC940.14212921662609432No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC930.14061720368326353No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC910.13759317779760194No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA890.13456915191194038No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCACAATGGAGGAAACTCTGAT820.12398506131212482No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC820.12398506131212482No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT810.12247304836929404No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT790.11944902248363246No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA770.11642499659797088No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA770.11642499659797088No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT740.1118889577694785No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA740.1118889577694785No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT740.1118889577694785No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC710.10735291894098614No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT710.10735291894098614No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT700.10584090599815535No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC700.10584090599815535No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA690.10432889305532457No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC680.10281688011249375No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT670.10130486716966297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTAA600.0273.1612245
TTGATAA159.795225E-6273.1612245
ATATTAA159.795225E-6273.1612245
TAAGGCA350.0273.1612245
ATCCTAT800.0273.16116245
CTGGTAA100.00106322273.16116245
AAAACTT950.0273.16116245
GTGGTCA100.00106322273.16116245
AGATTAA5550.0270.70026245
TGAAAGG6200.0259.9437245
ACGGTTA10750.0245.20981245
TACGGGT11500.0244.853263
GGGAGGC12600.0244.853246
CCTACGG64750.0244.47511
GAGGCAG7950.0243.313288
GTGGCTG7350.0243.187588
TGGCTGC7350.0243.187589
GGGGCTG13900.0243.091728
GGGCTGC13900.0243.091729
CTACGGG65050.0242.971222
Produced by FastQC (version 0.11.5)