FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94295
Sequences flagged as poor quality0
Sequence length204-251
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG50795.3862877140887635No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG34953.7064531523410578No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG28913.065910175513018No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG25522.7064001272601943No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG24562.6045919720027575No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG22972.4359722148576277No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG21862.318256535341216No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG19202.036163105148735No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16201.7180126199692454No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG15611.6554430245506124No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG13711.4539477172702688No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13311.4115276525796703No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG13251.4051646428760804No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12401.3150220054085582No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG12251.2991144811495836No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG12011.2736624423352245No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG11751.2460894002863354No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG10411.1039821835728298No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9531.0106580412535129No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9030.9576329603902647No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG8280.8780953390953922No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7610.8070417307386394No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG7150.758258656344451No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG6960.7381091256164165No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG6910.7328066175300917No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6790.7200805981229121No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6730.7137175884193224No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG6520.691447054456758No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG6170.6543294978524843No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6100.6469059865316295No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG6070.6437244816798346No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG5960.6320589638899199No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5680.6023649186065009No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG5670.6013044169892359No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG5340.566307863619492No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG5130.5440373296569277No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG4640.4920727504109444No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4590.4867702423246196No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4510.4782862293864998No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG4380.4644997083620553No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG4200.44541067925128586No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG4180.44328967601675595No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4170.442229174399491No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4050.42950315499231134No Hit
GACTACTGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG3880.4114746274988069No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3870.410414125881542No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG3700.3923855983880375No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3590.3807200805981229No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3450.3658730579564134No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3360.35632854340102865No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3330.3531470385492338No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG3310.35102603531470383No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCAG3300.3499655336974389No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG3190.3383000159075243No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3190.3383000159075243No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2990.31708998356222495No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG2950.31284797709316503No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG2900.3075454690068402No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2890.30648496738957526No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2870.30436396415504535No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2840.30118245930325044No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG2830.3001219576859855No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG2700.28633543666154093No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2650.2810329285752161No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2590.2746699188716263No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2580.27360941725436133No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG2490.2640649026989766No Hit
GACTACAGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG2440.25876239461265177No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2310.24497587358820722No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCAG2280.24179436873641233No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2220.23543135903282253No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2210.23437085741555755No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2160.2290683493292327No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA2120.22482634286017286No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2110.22376584124290788No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2110.22376584124290788No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2080.220584336391113No Hit
GACTACTCGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG2040.21634232992205316No Hit
GACTACCGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG1970.20891881860119837No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1910.20255580889760857No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1900.2014953072803436No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1880.19937430404581366No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1870.1983138024285487No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1830.19407179595948884No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1790.18982978949042897No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1760.1866482846386341No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCAG1710.18134577655230924No Hit
GACTACTAGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG1710.18134577655230924No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1660.17604326846598442No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1590.16861975714512964No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1580.1675592555278647No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCAG1580.1675592555278647No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1570.1664987539105997No Hit
GACTACAAGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG1560.16543825229333475No Hit
GACTACCCGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG1540.1633172490588048No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1500.15907524258974495No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1470.15589373773795004No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1470.15589373773795004No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1440.15271223288615515No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1430.1516517312688902No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1420.15059122965162522No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1420.15059122965162522No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCATGAG1420.15059122965162522No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1410.14953072803436024No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1380.14634922318256535No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA1360.14422821994803542No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG1330.14104671509624053No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1330.14104671509624053No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCAG1310.13892571186171057No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1310.13892571186171057No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1280.13574420700991569No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1280.13574420700991569No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1260.13362320377538578No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1250.1325627021581208No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1250.1325627021581208No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1220.12938119730632588No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCAG1140.12089718436820616No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA1130.11983668275094118No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA1120.11877618113367625No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA1120.11877618113367625No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1120.11877618113367625No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCCGGCTTTCGCACTTCAG1100.1166551778991463No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1070.1134736730473514No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1060.11241317143008643No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1050.11135266981282146No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1010.1071106633437616No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCAG1000.10605016172649663No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG1000.10605016172649663No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG990.10498966010923166No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG970.10286865687470173No Hit
GACTACCAGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG970.10286865687470173No Hit
GACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCATGAG950.1007476536401718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGA251.1659722E-9258.38052245
ATCCAGG100.0012569943258.38052245
CACCGGA100.0012569943258.38052245
AACCCGG151.2250994E-5258.38052245
ATCAGGA201.1918564E-7258.38052245
TACCGGA100.0012569943258.38052245
ATACGGA151.2250994E-5258.38052245
ATTCGGG251.1659722E-9258.38052245
TCCCGGA100.0012569943258.38052245
ATCCGTA201.1918564E-7258.38052245
ATCCGGG301.0913936E-11258.38052245
ATTCAGG151.2250994E-5258.38052245
ATCCGGA32800.0256.4112245
TTCCGGA19250.0253.0116245
AGGGGTC100.001475005244.94757
TGGTATC100.001475005244.94759
AGGGTCT201.5558726E-7244.94758
AAGGGTC100.001475005244.94757
TATTGGG100.001475005244.94754
AAGGGGA100.001475005244.94757
Produced by FastQC (version 0.11.5)