FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46556
Sequences flagged as poor quality0
Sequence length51-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13482.8954377523842254No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11692.510954549359911No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA9562.0534410172695248No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC8231.7677635535698943No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA8181.757023799295472No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7831.6818455193745168No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC7631.638886502276828No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7561.6238508462926369No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT7381.585187730904717No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6401.3746885471260417No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6231.3381733825930062No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5791.2436635449780908No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5181.11263854283014No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4991.0718274765873357No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4871.0460520663287225No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4831.0374602629091847No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4370.9386545235845004No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4220.9064352607612338No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA4100.8806598505026205No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4050.8699200962281982No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA4050.8699200962281982No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4030.8656241945184294No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4010.8613282928086605No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3930.8441446859695851No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT3820.8205172265658561No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3750.8054815705816651No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3670.7882979637425895No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3640.7818541111779362No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3590.771114356903514No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3440.7388950940802474No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3200.6873442735630209No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT3190.6851963227081365No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3190.6851963227081365No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2910.6250536987713721No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2790.5992782885127588No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2780.5971303376578744No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2770.59498238680299No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2630.5649110748346078No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA2600.5584672222699544No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2470.5305438611564567No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT2420.5198041068820346No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA2400.5155082051722657No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2370.5090643526076123No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT2270.48758484405876795No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2260.48543689320388345No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2250.48328894234899905No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2200.4725491880745769No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2200.4725491880745769No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2180.468253286364808No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2180.468253286364808No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2130.45751353209038575No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA2000.429590170976888No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT1970.4231463184122347No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1960.4209983675573503No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1950.41885041670246587No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1950.41885041670246587No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1910.41025861328292806No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1900.40811066242804367No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1870.4016668098633903No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1860.3995188590085059No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1840.395222957298737No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1750.375891399604777No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1730.3715954978950082No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1650.3544118910559326No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1570.33722828421685713No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1510.3243405790875505No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1500.32219262823266603No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT1400.30071311968382164No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1390.2985651688289372No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1380.2964172179740528No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1380.2964172179740528No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1360.29212131626428384No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1340.287825414554515No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1330.28567746369963054No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1310.2813815619898617No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1260.2706418077154395No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1200.25775410258613285No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1190.2556061517312484No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1180.25345820087636395No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1160.24916229916659505No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT1160.24916229916659505No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1110.23842254489217285No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1110.23842254489217285No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1110.23842254489217285No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT1100.23627459403728845No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1100.23627459403728845No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1100.23627459403728845No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1060.22768279061775065No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1050.2255348397628662No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1040.2233868889079818No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1030.22123893805309736No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1010.21694303634332848No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1010.21694303634332848No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA980.21049918377867516No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC980.21049918377867516No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA970.2083512329237907No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA960.20620328206890629No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA950.20405533121402183No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA940.20190738035913736No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC930.19975942950425296No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA920.1976114786493685No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT920.1976114786493685No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA900.1933155769395996No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC890.1911676260847152No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC880.18901967522983074No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA880.18901967522983074No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT880.18901967522983074No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC870.1868717243749463No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC870.1868717243749463No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC860.18472377352006186No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT810.17398401924563967No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA780.16754016668098634No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC760.16324426497121747No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA760.16324426497121747No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA750.16109631411633302No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT750.16109631411633302No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA740.15894836326144857No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA740.15894836326144857No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA730.15680041240656414No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC730.15680041240656414No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT730.15680041240656414No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA730.15680041240656414No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA680.14606065813214192No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC680.14606065813214192No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT680.14606065813214192No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA670.1439127072772575No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT670.1439127072772575No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC670.1439127072772575No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA650.1396168055674886No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC650.1396168055674886No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA620.1331729530028353No Hit
CCTACGGGCGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA620.1331729530028353No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC620.1331729530028353No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC620.1331729530028353No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC620.1331729530028353No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA610.13102500214795085No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT600.12887705129306642No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGAA600.12887705129306642No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGCGAAAGCCCGAT600.12887705129306642No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT590.12672910043818197No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC560.12028524787352865No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA560.12028524787352865No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGGACAATGGGCGAAAGCCCGAT550.11813729701864423No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC550.11813729701864423No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA540.11598934616375976No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC520.1116934444539909No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC520.1116934444539909No Hit
CCTACGGGGGGCTGCAGCTAAGAATATTCCGCAATGGACGGAAGTCTGAC520.1116934444539909No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC510.10954549359910644No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA500.107397542744222No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT500.107397542744222No Hit
CCTACGGGGGGCAGCAGCTAAGAATATTCCGCAATGGACGGAAGTCTGAC500.107397542744222No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC490.10524959188933758No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA480.10310164103445314No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT480.10310164103445314No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT480.10310164103445314No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA470.10095369017956868No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA470.10095369017956868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCAA109.281351E-4285.72397245
ATCTAAA109.281351E-4285.72397245
GCGGTGG207.166636E-8285.72397245
GCGGTCG109.281351E-4285.72397245
GCGGCAT158.1658945E-6285.72397245
GGCGAGG109.281351E-4285.72397245
GAGTATA109.281351E-4285.72397245
TGAATGG109.281351E-4285.72397245
CCCTTAA207.166636E-8285.72397245
ACGGTTA3300.0272.7365245
TCGGTAA1750.0269.39688245
AGATTAA1450.0266.01886245
TGAAAGG5600.0257.6618245
GAAAATG2400.0255.96106245
CGTATTA900.0253.97684245
AATCCCA400.0250.00847245
CCTACGT100.0014788967244.599371
AGGCAGC5400.0244.599359
GGGCTGC10450.0244.599359
CCTACGG45700.0243.528881
Produced by FastQC (version 0.11.5)