FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61273
Sequences flagged as poor quality0
Sequence length35-251
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT45237.381717885528699No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT40206.560801658152856No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT30534.9826187717265356No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT24934.068676252182854No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT23503.8352945016565205No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT17762.898503419124247No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT15672.5574070145088377No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT15482.5263982504528912No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13952.2766960977918496No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13102.1379726796468264No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC12882.1020677949504676No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC11781.922543371468673No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8811.4378274280678276No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC8151.3301127739787508No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7901.2893117686419793No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7731.2615670850129748No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC7211.17670099391249No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC7051.1505883504969563No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA6521.0640902191830008No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA6211.0134969725654042No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5420.8845657957012062No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5380.8780376348473227No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5270.8600851924991432No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC5210.850292951218318No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4890.7980676643872505No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4270.6968811711520572No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA4030.6577122060287566No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3950.6446558843209896No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA3880.6332316028266937No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3450.5630538736474466No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3390.5532616323666215No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA3340.5451014312992672No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3060.49940430532208313No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2880.47002758147960766No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA2580.42106637507548184No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2500.408010053367715No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2340.38189740995218124No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2280.37210516867135607No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA2240.3655770078174726No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2240.3655770078174726No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2190.35741680675011833No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA2160.3525206861097057No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2010.32804008290764286No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1880.30682356013252166No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1850.3019274394921091No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1830.2986633590651674No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1760.2872390775708713No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1740.2839749971439296No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1620.26439051458227936No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1620.26439051458227936No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1430.23338175052633298No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1420.2317497103128621No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1390.22685358967244953No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1370.22358950924550783No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1370.22358950924550783No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1360.22195746903203697No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1290.21053318753774092No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1280.20890114732427006No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1280.20890114732427006No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1270.2072691071107992No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1250.2040050266838575No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1220.19910890604344492No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1160.18931666476261974No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1160.18931666476261974No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1090.1778923832683237No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1090.1778923832683237No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1040.16973218220096944No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1010.16483606156055686No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT990.16157198113361512No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC920.15014769963931912No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT890.14525157899890653No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC890.14525157899890653No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT880.14361953878543565No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC870.1419874985719648No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC860.14035545835849395No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC850.1387234181450231No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC840.13709137793155224No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT830.13545933771808139No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT820.13382729750461053No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC810.13219525729113968No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT790.12893117686419792No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC790.12893117686419792No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC780.12729913665072706No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT760.12403505622378536No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC740.12077097579684365No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA730.11913893558337277No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC710.11587485515643105No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC710.11587485515643105No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT690.11261077472948934No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA680.11097873451601849No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC660.10771465408907675No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT650.10608261387560589No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC650.10608261387560589No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA640.10445057366213503No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA630.10281853344866418No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA620.10118649323519333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAT350.0294.7699245
AAACCCG108.4610446E-4294.7699245
TCGGTAA350.0294.7699245
CTGATAA108.4610446E-4294.7699245
ACCTGGG108.4610446E-4294.7699245
TCTTAAG157.222283E-6294.7699245
AGATTAA3800.0287.01282245
ACGGTTA23450.0284.7138245
AAAACTT1000.0280.03143245
TGAAAGG5800.0279.5232245
CGTATTA500.0265.29294245
AATCCCA301.5679689E-9245.64159245
TTATTAA301.5679689E-9245.64159245
GAGGCAG6450.0244.719188
CCTACTG151.519316E-5244.719181
ACGGTGG100.0014778671244.719184
CCTACGG60050.0244.311661
GGGGCTG13200.0243.792248
GGGTGGC13200.0243.792246
CGGCTGC7100.0242.99589
Produced by FastQC (version 0.11.5)