FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72539
Sequences flagged as poor quality0
Sequence length50-251
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1087114.986421097616454No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1042514.371579426239677No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT64838.93726133528171No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT61948.538854960779718No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT51957.161664759646536No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT39365.426046678338549No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT37445.1613614745171565No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT34534.760197962475358No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT4670.6437916155447415No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4440.6120845338369705No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4300.5927845710583272No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4160.5734846082796841No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT3980.5486703704214285No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT3890.5362632514923007No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT3710.5114490136340452No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3340.46044196914763097No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3150.43424916251947226No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2800.38599925557286424No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2620.3611850177146087No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT2370.3267207984670315No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2340.3225850921573223No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT2280.31431367953790373No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2010.2770923227505204No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1940.2674423413611988No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT1920.2646852038213926No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1890.2605494975116833No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1770.24400667227284634No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1710.23573525965342781No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1700.2343566908835247No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1420.19575676532623829No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT1390.19162105901652904No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1390.19162105901652904No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1390.19162105901652904No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1380.19024249024662596No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT1330.18334964639711052No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1320.18197107762720743No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1310.18059250885730435No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1290.1778353713174982No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1280.17645680254759508No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT1270.175078233777692No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT1260.1736996650077889No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1210.16680682115827347No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1190.1640496836184673No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1150.15853540853885495No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT1150.15853540853885495No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1140.15715683976895187No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1050.1447497208398241No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1040.14337115206992102No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA800.11028550159224693No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGACGCAAGCCTGAT760.1047712265126346No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT760.1047712265126346No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGCGCAAGCCTGAT740.1020140889728284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAATT50.0066653881106.5486245
ACAGTCA50.0066653881106.5486245
AAACCTT50.0066653881106.5486245
TTGTTAA50.0066653881106.5486245
GGATGAT50.0066653881106.5486245
TGCATGG208.185452E-111106.5486245
AATCCCA300.01106.5486245
GCGACTT50.0066653881106.5486245
GCGGCGT50.0066653881106.5486245
GCGGTCG208.185452E-111106.5486245
GCGGCAT50.0066653881106.5486245
ACCTGGT50.0066653881106.5486245
ACACACA50.0066653881106.5486245
AAAAGTG50.0066653881106.5486245
GTCTCGT50.0066653881106.5486245
GCAGATT50.0066653881106.5486245
AAAACTA50.0066653881106.5486245
AAAATTT50.0066653881106.5486245
GTGGTTT50.0066653881106.5486245
GTGGCGT50.0066653881106.5486245
Produced by FastQC (version 0.11.5)