FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57319
Sequences flagged as poor quality0
Sequence length42-251
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT25214.398192571398664No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT23154.038800397773862No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT22883.991695598318184No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT21743.7928086672831No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT15712.7408014794396274No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT13992.440726460684939No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13452.346516861773583No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT13272.315113662136464No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13082.2819658402972833No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13082.2819658402972833No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT9771.7044958914147141No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT9461.6504126031507877No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT9281.619009403513669No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9151.5963293148868611No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC8701.5178213157940648No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8251.4393133167012684No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8191.4288455834888956No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC7871.373017673022907No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC5680.9909454107712976No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4880.851375634606326No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4860.8478863902022018No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4810.839163279191891No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4730.8252063015753939No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4460.778101502119716No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4280.7466983024825974No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4250.741464435876411No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3630.6332978593485581No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3450.6018946597114395No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3430.5984054153073152No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3390.5914269264990666No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3310.5774699488825694No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA3300.5757253266805074No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA3080.5373436382351402No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2950.5146635496083323No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2820.49198346098152446No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2780.4850049721732759No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2780.4850049721732759No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2740.4780264833650273No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2710.4727926167588409No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2650.462324883546468No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2630.4588356391423437No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2560.4466232837279087No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA2520.4396447949196602No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2510.43790017271759807No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2390.4169647062928523No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2310.40300772867635515No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2300.401263106474293No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2250.39253999546398227No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2190.3820722622516094No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2130.3716045290392365No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2070.3611367958268637No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA2040.3559029292206773No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA2040.3559029292206773No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1930.3367120849979937No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1930.3367120849979937No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1900.33147821839180724No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1880.32798897398768295No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1840.32101048517943437No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1840.32101048517943437No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1730.30181964095675085No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1730.30181964095675085No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1700.2965857743505644No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1670.29135190774437797No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1640.2861180411381915No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1550.2704164413196322No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1540.2686718191175701No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1520.2651825747134458No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1440.25122559709694864No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1430.2494809748948865No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1390.24250248608663794No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1380.24075786388457582No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1370.23901324168251364No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1340.23377937507632723No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC1310.22854550847014077No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1300.22680088626807865No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1300.22680088626807865No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1290.22505626406601648No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1290.22505626406601648No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC1280.22331164186395436No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1250.21807777525776795No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1250.21807777525776795No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1240.2163331530557058No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1230.21458853085364366No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1190.20761004204539507No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1180.20586541984333293No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1180.20586541984333293No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1160.20237617543920863No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1120.19539768663096005No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1110.19365306442889793No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1080.1884191978227115No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1080.1884191978227115No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1060.1849299534185872No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1040.18144070901446294No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1020.17795146461033864No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1020.17795146461033864No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1010.1762068424082765No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT1010.1762068424082765No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1000.17446222020621435No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1000.17446222020621435No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1000.17446222020621435No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC1000.17446222020621435No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA960.16748373139796577No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT930.16224986479177933No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT920.16050524258971718No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA890.15527137598353075No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC890.15527137598353075No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC880.15352675378146863No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT880.15352675378146863No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT880.15352675378146863No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA870.15178213157940648No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA860.15003750937734434No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT830.1448036427711579No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC820.14305902056909575No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA800.13956977616497146No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC790.13782515396290934No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT770.13433590955878505No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT730.12735742075053647No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA730.12735742075053647No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC720.12561279854847432No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC710.1238681763464122No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT700.12212355414435005No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA690.12037893194228791No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC660.11514506533610147No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT660.11514506533610147No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT650.11340044313403932No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC640.11165582093197718No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC630.10991119872991505No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC630.10991119872991505No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC620.1081665765278529No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA590.10293270992166646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAT108.4207224E-4295.22245
AAACTTG108.4207224E-4295.22245
ACTTTAA108.4207224E-4295.22245
GAGTATA157.175451E-6295.22245
CGTATTA108.4207224E-4295.22245
ACCCCGG108.4207224E-4295.22245
AAAGGCA108.4207224E-4295.22245
CTATTAA108.4207224E-4295.22245
AGATTAA1750.0278.35028245
ACGGTTA12650.0276.54996245
TGAAAGG9450.0276.47586245
AATCCCA750.0275.53867245
GAAAATG2300.0256.71304245
TACCCGG351.2732926E-11253.04572245
TGGAAGG301.551598E-9246.01668245
CCTACGG55650.0244.728641
TACGGGT11900.0244.728623
CTACGGT100.0014774482244.728622
GTGGCTG6800.0244.728628
GAGGCTG5650.0244.72868
Produced by FastQC (version 0.11.5)