FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48578
Sequences flagged as poor quality0
Sequence length35-251
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT15523.1948618716291324No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT13972.875787393470295No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10352.1305940960928815No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT9291.9123883239326445No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT8541.7579974474041749No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC6961.4327473341841985No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC6931.42657169912306No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6191.27423936761497No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5921.2186586520647207No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5731.1795462966775083No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5591.150726666392194No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5501.1321997612087777No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4991.0272139651694183No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4941.0169212400675203No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4931.0148626950471407No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4891.0066285149656222No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4620.9510477994153731No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC4610.9489892543949937No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4490.9242867141504385No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA4450.91605253406892No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4450.91605253406892No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4430.911935444028161No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA4380.9016427189262629No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4290.8831158137428465No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC4190.8625303635390507No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4100.8440034583556343No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4010.8254765531722179No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3970.8172423730906995No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3650.7513689324385524No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3540.7287249372143769No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3530.7266663921939973No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3350.6896125818271646No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT3320.6834369467660257No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3120.6422660463584339No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3120.6422660463584339No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3050.6278562312157767No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC2990.6155049610934991No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT2980.6134464160731194No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2920.601095145950842No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2810.5784511507266663No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA2790.5743340606859072No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2770.570216970645148No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2750.5660998806043889No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2730.5619827905636297No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2720.55992424554325No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2650.5455144304005929No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2640.5434558853802133No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2640.5434558853802133No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2620.5393387953394541No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2500.514636255094899No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2500.514636255094899No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC2490.5125777100745194No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2450.5043435299930009No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2450.5043435299930009No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA2410.4961093499114826No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA2330.4796409897484458No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC2320.4775824447280662No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT2310.47552389970768655No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2270.46728971962616817No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2260.46523117460578867No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2220.4569969945242703No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT2140.4405286343612335No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1870.3849479188109844No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1840.37877228374984556No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT1840.37877228374984556No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1820.3746551937090864No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1810.37259664868870684No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT1800.37053810366832723No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1770.36436246860718846No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT1770.36436246860718846No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1770.36436246860718846No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1750.36024537856642924No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1740.35818683354604963No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1630.3355428383218741No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1610.33142574828111493No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1610.33142574828111493No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1600.3293672032607353No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1590.3273086582403557No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1560.32113302317921694No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1480.30466466301618017No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1460.300547572975421No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1460.300547572975421No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1450.29848902795504134No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1420.2923133928939026No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1400.2881963028531434No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT1380.28407921281238424No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1370.28202066779200463No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1360.279962122771625No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1350.2779035777512454No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1350.2779035777512454No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT1310.26966939766972703No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1310.26966939766972703No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT1260.25937667256782904No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1240.2552595825270699No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1230.25320103750669026No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1230.25320103750669026No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1220.25114249248631065No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT1210.2490839474659311No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT1210.2490839474659311No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1180.24290831240479227No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1170.24084976738441272No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1120.23055704228251472No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1110.22849849726213514No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1100.22643995224175553No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1070.22026431718061676No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1050.21614722713985754No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1030.21203013709909835No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1010.20791304705833916No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA990.20379595701757997No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCGCAATGGGGGAAACCCTGAC960.1976203219564412No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC950.19556177693606158No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA910.1873275968545432No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA880.18115196179340443No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC870.17909341677302482No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT860.17703487175264523No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA850.17497632673226562No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT850.17497632673226562No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACTCTGAT840.17291778171188604No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA800.16468360163036766No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT780.16056651158960847No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA780.16056651158960847No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA780.16056651158960847No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCGCAATGGGGGAAACCCTGAC760.15644942154884928No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC750.15439087652846967No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT750.15439087652846967No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA750.15439087652846967No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA740.15233233150809009No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT740.15233233150809009No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA730.1502737864877105No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA710.1461566964469513No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC700.1440981514265717No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT680.1399810613858125No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT640.13174688130429413No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA640.13174688130429413No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT610.12557124624315533No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC610.12557124624315533No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA610.12557124624315533No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC600.12351270122277573No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT590.12145415620239614No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA570.11733706616163696No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT570.11733706616163696No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC570.11733706616163696No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA570.11733706616163696No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA560.11527852114125736No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC560.11527852114125736No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT550.11321997612087777No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT550.11321997612087777No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA530.10910288608011857No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT510.10498579603935938No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC500.10292725101897979No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT500.10292725101897979No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT490.10086870599860019No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT490.10086870599860019No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCGCAATGGGGGAAACCCTGAC490.10086870599860019No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAT107.089784E-4312.5921245
ATCCAAT107.089784E-4312.5921245
CGTATTA107.089784E-4312.5921245
AATCCCA155.702055E-6312.59207245
ATCTGGT303.6379788E-12312.59207245
AGTCAAA155.702055E-6312.59207245
TAAGGCA155.702055E-6312.59207245
ACGGTTA7350.0310.4656245
AGATTAA3250.0307.783245
GGATTAA2150.0298.05292245
TCGGTAA650.0288.54657245
TGAAAGG2400.0286.54276245
AAAACTT500.0281.33292245
ACGGGTA251.3922727E-7250.0737245
CTACGGT100.0014778991244.66532
TGGCTGC6300.0244.66539
TCTACGG151.518713E-5244.665281
AGGCTGC2850.0244.665289
GGCGGAT5650.0243.43456245
GGGCTGC9800.0243.4179
Produced by FastQC (version 0.11.5)