FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005307917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length249-251
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT35.263157894736842No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT23.508771929824561No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGCGCAAGCCTGAC23.508771929824561No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA11.7543859649122806No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAT11.7543859649122806No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACGGAAGGCTGAT11.7543859649122806No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGCAAGTCTGAC11.7543859649122806No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAACCCTGAC11.7543859649122806No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGGACAATGGACGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC11.7543859649122806No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGCAAGTCTGAC11.7543859649122806No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC11.7543859649122806No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGACGAAACCCTGAC11.7543859649122806No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11.7543859649122806No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.7543859649122806No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCCAGAGCCTGA11.7543859649122806No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGGACAATGGAGGAGACTCTGAT11.7543859649122806No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC11.7543859649122806No Hit
CCTACGGGGGGCTGCAGTCGGGAATCTTCCACAATGGACGAAAGTCTGAC11.7543859649122806No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGAGGAAACCCTGAT11.7543859649122806No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAAGCCTGAC11.7543859649122806No Hit
CCTACGGGGGGCTGCAGTGAGGAATTTTGGACAATGGGCGCGAGCCTGAA11.7543859649122806No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC11.7543859649122806No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT11.7543859649122806No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGTCAATGGGGGGAAACCTGAA11.7543859649122806No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACCAAGGTCTGAC11.7543859649122806No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAACCCTGAT11.7543859649122806No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGCGCAAGCCTGAC11.7543859649122806No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC11.7543859649122806No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAC11.7543859649122806No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC11.7543859649122806No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACGATGGGGGCAACCCTGAT11.7543859649122806No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGGACAATGGGGGAAACCCTGAC11.7543859649122806No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGGCAATGGGGGCAACCCTGAC11.7543859649122806No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGGAAGTCTGAC11.7543859649122806No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAC11.7543859649122806No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAA11.7543859649122806No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGGACAATGGGCGGAAGCCTGAC11.7543859649122806No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT11.7543859649122806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)