FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length249-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG23.508771929824561No Hit
GACTACCGGGGTATCTCATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG11.7543859649122806No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTCGCTTTCGCGCCTCAG11.7543859649122806No Hit
GACTACTCGGGTATCTAATCCTATTTGCTCCCCAAGCTTTCGACTATCAG11.7543859649122806No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAACATGAG11.7543859649122806No Hit
GACTACTCGGGTATCTAATCCTGTCCGCTCCCCATGCTTTCGCTTCTCAG11.7543859649122806No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTTAG11.7543859649122806No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG11.7543859649122806No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACCTGAG11.7543859649122806No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA11.7543859649122806No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAG11.7543859649122806No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATCAG11.7543859649122806No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA11.7543859649122806No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCTCGCGTTCGCGCCTCAG11.7543859649122806No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAA11.7543859649122806No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCATGAA11.7543859649122806No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCATCAG11.7543859649122806No Hit
GGTTACAAGTGTAACTAATACTGTTTGCTAACCACGCTTGCGAGCCATTT11.7543859649122806No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGATCCTCAG11.7543859649122806No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG11.7543859649122806No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11.7543859649122806No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTGAG11.7543859649122806No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCTGGCTTTCGCGCCTCAG11.7543859649122806No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA11.7543859649122806No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAG11.7543859649122806No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGGGCATGAA11.7543859649122806No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG11.7543859649122806No Hit
GACTACTGGGGTATCTAATCCTGTCCGATACCCGCACCTTCGAGCCTCAG11.7543859649122806No Hit
GACTACCCGGGTATCTAATCCAGTTTGCTACCCTGGCTTTCGCGCCTCAG11.7543859649122806No Hit
GACTACACGGGTATCTAATCCTGATTGCTCCCCAAGCTTTCGCACTTCAG11.7543859649122806No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATGAA11.7543859649122806No Hit
GACCCCCAGTTTCTCTAATCCTGTTCGCTCCCCCCGCTTTCGTGCATGAA11.7543859649122806No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACATCAG11.7543859649122806No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG11.7543859649122806No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATTCTTTCGCGTATGAG11.7543859649122806No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGATTCTCAG11.7543859649122806No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG11.7543859649122806No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCAAGCTTTCGCACTTCAG11.7543859649122806No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCCTCAG11.7543859649122806No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA11.7543859649122806No Hit
GACTACAAGGGTAGCTAATCCTGTTCGCTCCCCCTGCTTTCGCTCCTCAG11.7543859649122806No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG11.7543859649122806No Hit
GACTACCAGTGTATCTAATCCTGTTTGCTCCCCCCGCTTTCACGCCTCAG11.7543859649122806No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG11.7543859649122806No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG11.7543859649122806No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG11.7543859649122806No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCAAGCTTTCGAGCTTCAG11.7543859649122806No Hit
GACTACCCGTGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAT11.7543859649122806No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCGCTTCTCAG11.7543859649122806No Hit
GACGACCGGGGTATCTAATCCTGTTTGATCCCCTAGCTTTCGCACCTCAG11.7543859649122806No Hit
GACTACAGGTGTATCTAATCCTGTTTGCTACCCACGCTTTAGATCATGAA11.7543859649122806No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGCCTCTCAG11.7543859649122806No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG11.7543859649122806No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCTTCAG11.7543859649122806No Hit
TACTACCGTGGTCTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA11.7543859649122806No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11.7543859649122806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAAG50.0245.000023
AATCCGG50.0245.00002245
GGGTAGC50.0245.000029
AGGGTAG50.0245.000028
AAGGGTA50.0245.000027
TACAAGG50.0245.000024
ACAAGGG50.0245.000025
CAAGGGT50.0245.000026
ACTACAA50.0245.000022
GACTACA50.0245.000021
Produced by FastQC (version 0.11.5)