FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length249-251
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT35.555555555555555No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT23.7037037037037033No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC23.7037037037037033No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC23.7037037037037033No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGGCAATGGGCGCAAGCCTGAT11.8518518518518516No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAC11.8518518518518516No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGTCTGAC11.8518518518518516No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC11.8518518518518516No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGGACAATGGGCGGAAGCCTGAA11.8518518518518516No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT11.8518518518518516No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11.8518518518518516No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC11.8518518518518516No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACGCAAGCCTGAT11.8518518518518516No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT11.8518518518518516No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGACTGAT11.8518518518518516No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACGCAAGCCTGAT11.8518518518518516No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAC11.8518518518518516No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAACCCTGAT11.8518518518518516No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.8518518518518516No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGACAATGGGCGAAAGTCTGAA11.8518518518518516No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTGGACAATGGGCGCAAGCCTGAT11.8518518518518516No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCACAATGGGCGCAAGCCTGAT11.8518518518518516No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC11.8518518518518516No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC11.8518518518518516No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCCCAACCCTGAA11.8518518518518516No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAGAGCCTGAA11.8518518518518516No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT11.8518518518518516No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGAAACCCTGAC11.8518518518518516No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGAGGAATCCTGAC11.8518518518518516No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA11.8518518518518516No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTCGGCAATGGGGGCAAGCCTGAA11.8518518518518516No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT11.8518518518518516No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGGACAATGGACGAAAGTCTGAC11.8518518518518516No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC11.8518518518518516No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11.8518518518518516No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAC11.8518518518518516No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCTCAATGGGCGAAACCCTGAA11.8518518518518516No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGGAACCCTGAC11.8518518518518516No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT11.8518518518518516No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGTCTGAC11.8518518518518516No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTACACAATGGGCGCAAGCCTGAC11.8518518518518516No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAC11.8518518518518516No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT11.8518518518518516No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC11.8518518518518516No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11.8518518518518516No Hit
CCTACGGGCGGCAGCAGTAGGGAATATTCCGCAATGGGGGAAACCCTGAT11.8518518518518516No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCGCAATGGAGGGAAGCCTGAC11.8518518518518516No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGCCTGAC11.8518518518518516No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCGCAATGGAGGAAACCCTGAA11.8518518518518516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGG50.0245.000021
CGGGGGG50.0245.000025
TACGGGG50.0245.000023
GCGGTTT50.0245.00002245
GGGGGGC50.0245.000026
GGGGGCT50.0245.000027
ACGGGGG50.0245.000024
CTACGGG50.0245.000022
GGGGCTG50.0245.000028
GGGCTGC50.0245.000029
Produced by FastQC (version 0.11.5)