FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length248-251
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA35.555555555555555No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG11.8518518518518516No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCTTCAG11.8518518518518516No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGAGCATCAA11.8518518518518516No Hit
GACTACTGGGGTAGCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAA11.8518518518518516No Hit
GACTACAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAAC11.8518518518518516No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAACCTGAG11.8518518518518516No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTACCCAGGCTTTCGCGCATCAG11.8518518518518516No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCCGCCGAGCATGAA11.8518518518518516No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAG11.8518518518518516No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG11.8518518518518516No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA11.8518518518518516No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCAGGCTTTCGCGCCTCAG11.8518518518518516No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA11.8518518518518516No Hit
GACTACTAGGGTATCTAATCCTGTTCGATCCCCATACTTTCGATTCTCAG11.8518518518518516No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCAGGCTTTCGAGCATGAA11.8518518518518516No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG11.8518518518518516No Hit
GACTACAAGGGTATCTATTCCTGTTTGCTACCCACGCTTTCGGGCCAGAA11.8518518518518516No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG11.8518518518518516No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG11.8518518518518516No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG11.8518518518518516No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCACCTCAG11.8518518518518516No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACTTCAG11.8518518518518516No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATGAA11.8518518518518516No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGAGCATGAA11.8518518518518516No Hit
GACTACCAGGGTATCTAATCCTGTTCGTTACCCGCACCTTCGGGCTTCAA11.8518518518518516No Hit
GACTACTAGGGTCGCTAATCCAGTTCGATCCCCACGCTTTCGCTTCTCAG11.8518518518518516No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATGAG11.8518518518518516No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCCTGAA11.8518518518518516No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCGCACCTTCGAGCTTAAG11.8518518518518516No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTGCTCAG11.8518518518518516No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGAG11.8518518518518516No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACTTGAG11.8518518518518516No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATGAG11.8518518518518516No Hit
GACTACCCGGGGATCTAATCCTGTTTGCTCCCCACGCTTTCGTCTCTCAG11.8518518518518516No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCATCAG11.8518518518518516No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTTCTCAG11.8518518518518516No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG11.8518518518518516No Hit
GACTACTGTGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGCACCTGAG11.8518518518518516No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACGCACGCTTTCGAGCCTGAG11.8518518518518516No Hit
GACTACTCGGGTTTCTAATCCGGTTTGCTACCCACGCTTTCGTGCCTGAA11.8518518518518516No Hit
GCCTACTCGGGTATCTCATCCTGTTCTATCCCCGCACTTTCGAGCATCAC11.8518518518518516No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG11.8518518518518516No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACTTGAG11.8518518518518516No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11.8518518518518516No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA11.8518518518518516No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG11.8518518518518516No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTCCCTCAT11.8518518518518516No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG11.8518518518518516No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG11.8518518518518516No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG11.8518518518518516No Hit
GACTACTAGTGTATCTAATCCTGTTTGCTCTCCCCGCCTTCGCGCATTAG11.8518518518518516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATC50.0245.000029
CTGGGGT50.0245.000026
TGGGGTA50.0245.000027
TACTGGG50.0245.000024
GGGGTAT50.0245.000028
ATTCCGA50.0245.00002245
ACTGGGG50.0245.000025
CTACTGG50.0245.000023
ACTACTG50.0245.000022
GACTACT50.0245.000021
Produced by FastQC (version 0.11.5)