FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005307960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93194
Sequences flagged as poor quality0
Sequence length247-251
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG35953.8575444771122602No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG24372.614975212996545No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG21082.261948194089748No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG17911.9217975406142027No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG17291.8552696525527392No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG17001.8241517694272162No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG15461.6589050797261626No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG14411.5462368822027168No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG14231.5269223340558404No Hit
GACTACCCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG12571.348799278923536No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG11871.2736871472412388No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11561.2404232032105071No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG11161.1975019851063373No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG9341.0022104427323648No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9290.9968452904693436No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9260.9936261991115308No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG9050.9710925596068416No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG8860.9507049810073611No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8640.9270983110500677No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG8610.9238792196922548No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG8090.8680816361568342No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG8000.8584243620833959No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG7890.8466210271047492No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7610.8165761744318304No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG7590.8144301135266218No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA7150.7672167736120351No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6760.7253685859604696No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG6220.6674249415198403No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6040.6481103933729639No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG5970.6405991802047343No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG5900.6330879670365045No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5760.618065540700045No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5420.5815825053115007No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5410.5805094748588966No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5210.5590488658068116No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG5180.555829774448999No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4880.5236388608708715No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA4650.4989591604609739No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4650.4989591604609739No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG4570.49037491684013995No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4540.48715582548232716No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4530.48608279502972296No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG4470.4796446123140975No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG4450.477498551408889No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4410.47320642959847203No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG4340.4656952164302423No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4070.4367233942099277No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4040.43350430285211494No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3960.42492005923128096No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA3780.4056055110844046No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3730.4002403588213833No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3660.3927291456531536No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA3510.37663368886408993No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG3350.35946520162242207No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3260.3498079275489838No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA3220.34551580573856683No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG3200.34336974483335836No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3120.33478550121252443No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG3050.3272742880442947No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG3040.32620125759169044No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3040.32620125759169044No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2970.3186900444234607No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG2950.31654398351825225No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2950.31654398351825225No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2920.3133248921604395No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2880.3090327703500225No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2880.3090327703500225No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2850.3058136789922098No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2840.3047406485396056No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2540.27254973496147816No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2510.2693306436036655No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG2400.25752730862501877No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2300.2467970040989763No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG2160.23177457776251692No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG2150.23070154730991266No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2120.22748245595209993No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2110.2264094254994957No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2070.22211730368907867No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2060.22104427323647444No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG2030.2178251818786617No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1990.21353306006824474No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1930.20709487735261928No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1880.20172972508959805No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1860.19958366418438958No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1850.19851063373178532No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1840.19743760327918108No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1840.19743760327918108No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1820.1952915423739726No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG1760.1888533596583471No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1730.18563426830053437No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1720.18456123784793013No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG1710.18348820739532587No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG1700.18241517694272164No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1700.18241517694272164No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG1680.18026911603751314No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1680.18026911603751314No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1680.18026911603751314No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1650.1770500246797004No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1630.1749039637744919No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1630.1749039637744919No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1620.17383093332188768No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG1590.17061184196407495No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1560.16739275060626221No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1550.16631972015365798No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1530.16417365924844946No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1510.16202759834324099No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1490.1598815374380325No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1480.15880850698542825No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1460.15666244608021976No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1420.1523703242698028No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1410.15129729381719853No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1390.14915123291199003No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG1390.14915123291199003No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1360.1459321415541773No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1350.14485911110157307No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG1330.14271305019636457No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1300.13949395883855184No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG1300.13949395883855184No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1280.13734789793334334No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1260.13520183702813485No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1210.12983668476511365No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1200.12876365431250938No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG1200.12876365431250938No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1190.12769062385990515No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1190.12769062385990515No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1150.12339850204948816No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1150.12339850204948816No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1120.12017941069167543No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1120.12017941069167543No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1100.11803334978646694No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1090.1169603193338627No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1080.11588728888125846No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCTCAG1080.11588728888125846No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1060.11374122797604996No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1060.11374122797604996No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1060.11374122797604996No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG1060.11374122797604996No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG1040.11159516707084147No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1020.10944910616563297No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1020.10944910616563297No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG990.10623001480782024No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG990.10623001480782024No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG980.105156984355216No Hit
GACTACTGGGGTATCTAATCCTGTTCGATCCCCACACTTTCGCATCTCAG980.105156984355216No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG970.10408395390261176No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG960.10301092345000751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)