FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69374
Sequences flagged as poor quality0
Sequence length42-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG40695.8653097702309225No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG27053.8991553031395045No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG19192.766165998789172No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG18902.72436359443019No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG18642.686885576729034No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG17302.493729639346153No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG16392.3625565773921062No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG13351.924352062732436No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12291.7715570674892611No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG10301.4847060858534897No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG10161.4645256147836365No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9611.3852451927234988No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9391.3535330238994436No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8701.2540721307694525No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8471.2209184997261222No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7491.0796552022371495No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7451.0738893533600484No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6710.9672211491336812No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG6590.9499236025023785No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6500.9369504425289013No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5840.8418139360567358No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG5630.811543229451956No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5320.7668579006544238No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG5300.7639749762158734No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5100.7351457318303687No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5060.7293798829532677No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4980.7178481851990659No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG4800.6919018652521117No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG4760.6861360163750109No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG4690.6760457808400842No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4570.6587482342087814No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4530.6529823853316805No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4360.6284775276040014No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4300.6198287542883502No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG4180.6025312076570473No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4150.5982068209992216No Hit
GACTACCCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG4020.5794678121486435No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3840.5535214922016894No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3750.5405483322282123No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3490.5030703145270562No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3230.46559229682590025No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG3060.44108743909822123No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG2990.4309972035632946No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2980.42955574134401936No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2900.41802404358981754No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2880.415141119151267No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2780.40072649695851476No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2750.396402110300689No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2750.396402110300689No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2700.3891947992043128No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG2690.3877533369850376No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2650.3819874881079367No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2610.3762216392308358No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2540.36613140369590913No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2340.33730215931040447No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2340.33730215931040447No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2330.33586069709112926No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG2240.3228875371176521No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG2190.315680226021276No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2140.3084729149248998No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG2100.3027070660477989No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG2080.2998241416092484No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA2080.2998241416092484No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2060.296941217170698No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1970.28396805719722085No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1960.28252659497794563No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1950.2810851327586704No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1880.27099489722374376No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1820.26234612390809237No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1810.26090466168881715No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1750.25225588837316576No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1750.25225588837316576No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1660.23928272839968864No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1560.22486810620693634No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATAAA1530.22054371954911062No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1490.21477787067200968No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA1480.21333640845273444No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1470.21189494623345922No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1460.21045348401418396No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1430.2061290973563583No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1410.20324617291780783No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1410.20324617291780783No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1360.19603886182143165No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1360.19603886182143165No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1360.19603886182143165No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1330.19171447516360596No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1330.19171447516360596No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1330.19171447516360596No Hit
GACTACTGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG1310.1888315507250555No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1310.1888315507250555No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1280.1845071640672298No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1270.18306570184795457No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1230.17729985297085363No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1210.17441692853230317No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG1210.17441692853230317No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG1210.17441692853230317No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1200.17297546631302793No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1200.17297546631302793No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1180.17009254187447748No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1160.167209617435927No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1140.16432669299737654No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1120.16144376855882606No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATAAA1070.1542364574624499No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1040.1499120708046242No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1040.1499120708046242No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA1030.148470608585349No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCAG1010.1455876841467985No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG980.14126329748897282No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA960.13838037305042236No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG960.13838037305042236No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG960.13838037305042236No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG950.1369389108311471No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA950.1369389108311471No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG940.13549744861187188No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG930.13405598639259664No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG920.13261452417332142No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG880.1268486752962205No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG870.12540721307694525No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA830.11964136419984432No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCACTTCAG830.11964136419984432No Hit
GACTACAGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG820.1181999019805691No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA820.1181999019805691No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG810.11675843976129387No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG810.11675843976129387No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG790.1138755153227434No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATAAA790.1138755153227434No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATAAA780.11243405310346817No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG770.11099259088419293No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG760.1095511286649177No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA760.1095511286649177No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG760.1095511286649177No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATAAA740.10666820422636722No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG740.10666820422636722No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG730.10522674200709198No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG730.10522674200709198No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG730.10522674200709198No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG720.10378527978781675No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG720.10378527978781675No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG710.10234381756854152No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG710.10234381756854152No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG710.10234381756854152No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG700.1009023553492663No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAA700.1009023553492663No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAG700.1009023553492663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAG350.0252.80656245
AATCCGA100.0013410752252.80655245
TTCGTTT100.0013410752252.80655245
ATTCGGA201.3263525E-7252.80655245
ATCCGGG151.3350269E-5252.80655245
ATCCGGA11150.0248.27191245
TACAAGG6700.0244.969014
CAAGGGT6750.0244.969016
GGGCATC151.5140044E-5244.9699
GTGTATC100.0014737791244.9699
CTGGGGG100.0014737791244.9696
GAGTATC100.0014737791244.9699
GGAGTAT100.0014737791244.9698
TACTAGG7000.0244.9694
ACTACCG9100.0244.9692
GACTAAA100.0014737791244.9691
TACTGGG14850.0244.14424
CTACTGG14850.0244.14423
ACTGGGG14750.0244.13865
CTGGGGT14650.0244.132926
Produced by FastQC (version 0.11.5)