FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005307969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124781
Sequences flagged as poor quality0
Sequence length35-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT32012.565294395781409No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT30692.4595090598728975No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC26792.14696147650684No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC25132.0139284025612874No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA23421.87688830831617No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA21051.6869555461167967No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT19281.5451070275122014No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT18001.4425273078433416No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT16211.2990759811189203No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC16021.283849303980574No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC14081.1283769163574584No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA14071.1275755122975453No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13731.1003277742605044No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA13321.0674702078040728No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12971.0394210657071188No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC12881.0322084291679021No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT12420.9953438424119057No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT11340.9087922039413051No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC10770.863112172526266No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10660.8542967278672233No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA10270.8230419695306177No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC10000.8014040599129675No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT9760.7821703624750563No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT9750.7813689584151432No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT9500.7613338569173191No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC8880.7116468052027152No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT8530.6835976631057613No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8430.6755836225066316No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC8240.6603569453682853No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA7950.6371162276308091No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC7910.6339106113911573No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7700.617081126132985No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC7680.6154783180131591No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC7420.5946418124554219No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT7150.5730039028377718No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA7000.5609828419390773No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA6960.5577772256994253No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6840.5481603769804698No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT6640.5321322957822104No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6280.5032817496253437No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6060.4856508603072583No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5750.4608073344499563No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT5720.4584031222702174No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT5690.4559989100904785No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5660.4535946979107396No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT5650.4527932938508267No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5360.42955257611335057No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5280.4231413436340469No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA5160.4135244949150912No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4890.39188658529744114No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4800.3846739487582244No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA4660.37345429191944285No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4460.3574262107211835No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT4440.3558234026013576No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4230.33899391734318524No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4220.33819251328327227No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3910.3133489874259703No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3840.30773915900657955No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3770.30212933058718877No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3720.2981223102876239No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA3660.2933138859281461No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3340.26766895601093116No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA3320.2660661478911052No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3310.26526474383119225No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT3210.25725070323206256No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3010.2412226220338032No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC3010.2412226220338032No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2970.23801700579415136No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA2970.23801700579415136No Hit
CCTACGGGGGGCTGCAGTAGAGAATCTTCGGCAATGGGGGAAACCCTGAC2920.2340099854945865No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2910.23320858143467357No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2900.2324071773747606No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2880.23080436925493464No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC2880.23080436925493464No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2850.22840015707519573No Hit
CCTACGGGGGGCAGCAGTAGAGAATCTTCGGCAATGGGGGAAACCCTGAC2830.2267973489553698No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC2770.221988924595892No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC2760.221187520535979No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT2700.21637909617650122No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2660.21317347993684935No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA2660.21317347993684935No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2650.2123720758769364No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2600.20836505557737156No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2580.2067622474575456No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2540.20355663121789375No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA2500.20035101497824187No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2430.19474118655885111No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT2420.19393978249893812No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA2340.1875285500196344No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2320.18592574189980848No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2310.1851243378398955No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2270.18191872160024364No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA2200.17630889318085285No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2190.17550748912093989No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA2150.17230187288128804No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2140.17150046882137504No Hit
CCTACGGGGGGCTGCAGCTAAGAATATTCCGCAATGGACGAAAGTCTGAC2090.16749344852181022No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA2090.16749344852181022No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC2040.16348642822224538No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC2020.16188362010241944No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2010.16108221604250647No Hit
CCTACGGGGGGCTGCAGCTGAGAATCTTCCGCAATGGACGAAAGTCTGAC1990.15947940792268053No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1990.15947940792268053No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1990.15947940792268053No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1990.15947940792268053No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC1990.15947940792268053No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC1980.15867800386276756No Hit
CCTACGGGGGGCAGCAGCTGAGAATCTTCCGCAATGGACGAAAGTCTGAC1960.15707519574294163No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT1940.1554723876231157No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1920.15386957950328978No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA1910.1530681754433768No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1900.1522667713834638No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1840.14745834702398603No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1810.14505413484424712No Hit
CCTACGGGGGGCAGCAGCTAAGAATATTCCGCAATGGACGAAAGTCTGAC1790.14345132672442118No Hit
CCTACGGGGGGCTGCAGCTAAGAATATTCCGCAATGGACGGAAGTCTGAC1750.14024571048476933No Hit
CCTACGGGGGGCAGCAGCTAAGAATATTCCGCAATGGACGGAAGTCTGAC1740.13944430642485633No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1730.13864290236494337No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGCAAGCCTGAC1720.1378414983050304No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCACAATGGGCGAAAGCCTGAT1690.13543728612529152No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1690.13543728612529152No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGTCGGAAGACTGAT1630.1306288617658137No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA1630.1306288617658137No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1610.12902605364598776No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1590.12742324552616183No Hit
CCTACGGGCGGCTGCAGTAGAGAATCTTCGGCAATGGGGGAAACCCTGAC1570.12582043740633592No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1570.12582043740633592No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCGCAATGGGGGAAACCCTGAC1560.12501903334642292No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT1540.12341622522659701No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1530.12261482116668404No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1530.12261482116668404No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1530.12261482116668404No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1510.12101201304685809No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA1500.12021060898694513No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1480.1186078008671192No Hit
CCTACGGGAGGCAGCAGTAGAGAATCTTCGGCAATGGGGGAAACCCTGAC1470.11780639680720623No Hit
CCTACGGGTGGCTGCAGTAGAGAATCTTCGGCAATGGGGGAAACCCTGAC1470.11780639680720623No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT1460.11700499274729324No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT1450.1162035886873803No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1450.1162035886873803No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC1430.11460078056755435No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1420.11379937650764138No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1410.11299797244772843No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1400.11219656838781546No Hit
CCTACGGGAGGCAGCAGCTGAGAATCTTCCGCAATGGACGAAAGTCTGAC1400.11219656838781546No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGAGGAGACTCTGAT1400.11219656838781546No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1400.11219656838781546No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACAATGGTCGGAAGACTGAT1400.11219656838781546No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1390.11139516432790249No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1390.11139516432790249No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1380.1105937602679895No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1380.1105937602679895No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1370.10979235620807654No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1360.1089909521481636No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCGCAATGGGGGAAACCCTGAC1360.1089909521481636No Hit
CCTACGGGTGGCTGCAGCTGAGAATCTTCCGCAATGGACGAAAGTCTGAC1340.10738814402833764No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCACAATGGAGGAAACTCTGAT1320.10578533590851172No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT1320.10578533590851172No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC1260.1009769115490339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGT156.6866633E-6300.736245
GAAAATT156.6866633E-6300.736245
CGAATTA156.6866633E-6300.736245
ATCTGGT303.6379788E-12300.736245
ACGGATA156.6866633E-6300.736245
TAAGGCA156.6866633E-6300.736245
AATCCCG107.9799077E-4300.73596245
ATCTAAA107.9799077E-4300.73596245
CGCTTAA205.5933924E-8300.73596245
CTGATAA350.0300.73596245
TTGATAA107.9799077E-4300.73596245
CCCTTAA205.5933924E-8300.73596245
ATATCAA107.9799077E-4300.73596245
AAAACTT2850.0295.4599245
ACGGTTA17250.0286.7888245
TCGGTAA4850.0279.03336245
CGTATTA2300.0274.58502245
GCGGTGA7000.0259.92178245
GGATTAA2200.0259.7265245
GCGGTCG1000.0255.62555245
Produced by FastQC (version 0.11.5)