FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005307974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005307974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69279
Sequences flagged as poor quality0
Sequence length58-251
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG27653.991108416691927No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17972.5938596111375745No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA14602.1074207191212344No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG14072.030918460139436No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13311.9212171076372349No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13281.9168867910910954No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12351.7826469781607703No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12281.772542906219778No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA10531.5199411076949725No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG9331.3467284458493916No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8541.2326967768010508No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7681.108561035811718No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7501.0825791365348807No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA7131.0291718991324932No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG7031.0147375106453616No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA6961.0046334387043694No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA6910.9974162444608035No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6780.9786515394275321No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA6660.961330273242974No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG6630.9569999566968345No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG6430.9281311797225711No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG6110.8819411365637495No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6080.8776108200176099No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA5160.7448144459359979No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4870.7029547193233159No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4700.678416258895192No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4560.6582081150132074No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4490.6481040430722153No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG4490.6481040430722153No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4210.6076877553082464No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4000.5773755394852697No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3880.5600542733007116No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3880.5600542733007116No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3820.5513936402084325No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3790.547063323662293No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3570.5153076689906032No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3390.48932576971376607No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG3380.4878823308650529No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3230.4662307481343553No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3090.4460226042523709No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3070.4431357265549445No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA3050.44024884885751814No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG2990.4315882157652391No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2930.4229275826729601No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2920.42148414382424687No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2840.40993663303454153No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2620.37818097836285164No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2530.36519002872443307No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2510.3623031510270068No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2490.35941627332958037No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2440.3521990790860145No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA2380.34353844599373545No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2380.34353844599373545No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2350.339208129447596No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2300.33199093520403006No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2200.3175565467168983No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2140.30889591362461927No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2130.3074524747759061No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2100.3031221582297666No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2100.3031221582297666No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2070.2987918416836271No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG2070.2987918416836271No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1940.2800271366503558No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1870.2699230647093636No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1830.26414930931451086No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1700.24538460428123965No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1620.23383709349153425No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1610.23239365464282102No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1600.23095021579410788No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1600.23095021579410788No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1560.2251764603992552No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1530.22084614385311568No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1490.21507238845826296No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1430.2064117553659839No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA1400.2020814388198444No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1340.19342080572756537No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1330.19197736687885217No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1300.18764705033271267No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1280.1847601726352863No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1260.18187329493785995No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1250.18042985608914677No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1210.17465610069429408No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1080.1558913956610228No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1080.1558913956610228No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1070.15444795681230963No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1060.1530045179635965No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1000.1443438848713174No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1000.1443438848713174No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG980.1414570071738911No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG920.13279637408161205No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG910.13135293523289887No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG910.13135293523289887No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG840.12124886329190664No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG830.11980542444319346No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG800.11547510789705394No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG700.1010407194099222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)