FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005308015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40317
Sequences flagged as poor quality0
Sequence length35-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC12983.2194855768038293No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC12463.090507726269316No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA8752.170300369571149No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC8412.0859686980678123No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA7721.9148250117816308No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA7621.8900215789865318No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC7621.8900215789865318No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA7401.8354540268373143No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6261.5526948929731876No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5661.4038742962025945No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5031.2476126695934717No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5021.2451323263139618No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4751.1781630577671949No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA4711.1682416846491555No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4431.0987920728228786No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4361.0814296698663095No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA4181.0367834908351314No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4151.029342460996602No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3820.9474911327727757No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA3780.9375697596547362No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3720.9226876999776769No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3480.8631594612694397No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3320.8234739687972815No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3180.7887491628841432No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2910.7217798943373763No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2860.7093781779398268No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA2810.6969764615422774No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2760.684574745144728No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2620.6498499392315896No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2590.6424089093930601No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2530.6275268497160007No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA2520.6250465064364907No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2510.6225661631569809No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA2430.6027234169209018No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2240.5555968946102141No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2180.5407148349331548No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2080.5159114021380559No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC2040.5059900290200163No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2030.5035096857405065No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1950.4836669395044274No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1920.47622590966589773No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1900.471265223106878No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1890.4687848798273681No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1860.46134384998883843No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1840.45638316342981866No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1800.4464617903117791No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1750.4340600739142297No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1720.42661904407570006No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1660.41173698439864076No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1440.3571694322494233No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1430.3546890889699134No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1430.3546890889699134No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1370.3398070292928541No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1360.33732668601334426No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1340.3323659994543245No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1320.3274053128953047No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1310.3249249696157948No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGAA1280.3174839397772652No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1210.300121536820696No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGAA1180.29268050698216636No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1170.29020016370265644No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1160.28771982042314653No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1150.2852394771436367No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT1150.2852394771436367No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1120.27779844730510705No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1100.27283776074608723No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1090.2703574174665774No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1070.2653967309075576No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGATGGAAATCTGAA1070.2653967309075576No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1040.25795570106902793No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA1040.25795570106902793No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1020.25299501451000816No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1020.25299501451000816No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA990.24555398467147851No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGAA990.24555398467147851No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA970.24059329811245878No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA930.23067192499441921No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT920.22819158171490933No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGATGGAAATCTGAA900.22323089515588956No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT880.21827020859686982No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT870.2157898653173599No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA860.21330952203785003No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC850.21082917875834017No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC850.21082917875834017No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGAA850.21082917875834017No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGAA800.19842746236079073No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT770.19098643252226108No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT760.1885060892427512No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC760.1885060892427512No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC750.1860257459632413No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA750.1860257459632413No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGATGCAAATCTGAA740.18354540268373143No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT730.18106505940422155No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA730.18106505940422155No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGATGCAAATCTGAA720.17858471612471166No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC710.17610437284520178No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA700.1736240295656919No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC690.171143686286182No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA680.16866334300667213No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA680.16866334300667213No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT660.16370265644765236No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA660.16370265644765236No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC640.1587419698886326No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT640.1587419698886326No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGATGGAAATCTGAA640.1587419698886326No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA640.1587419698886326No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT640.1587419698886326No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGAA630.15626162660912268No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA620.15378128332961283No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGAGGGAACTCTGAT620.15378128332961283No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT620.15378128332961283No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA610.15130094005010294No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA600.14882059677059303No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT600.14882059677059303No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT590.14634025349108318No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGATGGAAATCTGAA590.14634025349108318No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC580.14385991021157327No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT570.14137956693206338No Hit
CCTACGGGGGGCAGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT560.13889922365255353No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATAGCCTGAA560.13889922365255353No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC550.13641888037304362No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA540.13393853709353373No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGATGCAAATCTGAA520.12897785053451397No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA520.12897785053451397No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGATGAAAATCTGAA510.12649750725500408No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCGCAATGGGGGAAACCCTGAC510.12649750725500408No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATAGCCTGAA500.12401716397549421No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC500.12401716397549421No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA490.12153682069598433No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGATGGAAATCTGAA490.12153682069598433No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGAGGGAACTCTGAT490.12153682069598433No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGATGAAAATCTGAA490.12153682069598433No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT490.12153682069598433No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGAA480.11905647741647443No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC480.11905647741647443No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT470.11657613413696456No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGAGGGAACTCTGAT470.11657613413696456No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA470.11657613413696456No Hit
CCTACGGGGGGCAGCAGCTAAGAATATTCCGCAATGGACGAAAGTCTGAC460.11409579085745467No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC460.11409579085745467No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT460.11409579085745467No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA460.11409579085745467No Hit
CCTACGGGGGGCTGCAGCTAAGAATATTCCGCAATGGACGAAAGTCTGAC460.11409579085745467No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA450.11161544757794478No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT440.10913510429843491No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC440.10913510429843491No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCGCAATGGGGGAAACCCTGAC440.10913510429843491No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT430.10665476101892502No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA430.10665476101892502No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT430.10665476101892502No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT420.10417441773941513No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA420.10417441773941513No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGAA420.10417441773941513No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGAA410.10169407445990526No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT410.10169407445990526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCAA100.0011083531269.25513245
ATGATAA100.0011083531269.25513245
ATCTAGT209.62591E-8269.25513245
GCGGTGG100.0011083531269.25513245
TGATTAA151.0342559E-5269.25513245
CCGTTAA100.0011083531269.25513245
ACCCTGG100.0011083531269.25513245
TTGATAA1150.0269.25513245
ACGGTTA800.0269.25513245
AGCTGGG100.0011083531269.25513245
GACTTAA100.0011083531269.25513245
TCTTAAG100.0011083531269.25513245
TAAGGCA151.0342559E-5269.25513245
CGATTAA350.0269.25513245
TTGTTAA1350.0259.28268245
AGATTAA4150.0249.79088245
AGGCAGC4450.0244.86859
CCTACGC100.0014733023244.868481
CTAACGG100.0014733023244.868482
AGGCTGC2650.0244.868489
Produced by FastQC (version 0.11.5)