FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005308050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56766
Sequences flagged as poor quality0
Sequence length42-251
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16812.9612796392206606No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG14122.4874044322305604No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11051.9465877461860972No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG10911.921925096008174No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG10421.8356058203854422No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7991.4075326780114856No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7951.4004862065320791No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7671.3511609061762322No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG7421.3071204594299404No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7371.2983123700806822No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG7341.2930275164711271No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG6991.2313708910263186No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG6961.2260860374167637No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6891.2137547123278019No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG6611.164429411971955No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG6481.1415283796638833No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5821.025261600253673No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5320.9371807067610893No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5090.896663495754501No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG5010.8825705527956875No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG4870.8579079026177642No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4720.8314836345699892No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4480.789204805693549No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4430.7803967163442906No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG4390.7733502448648839No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG4390.7733502448648839No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4360.7680653912553289No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG4090.7205017087693337No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG4010.7064087658105204No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3790.6676531726737835No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3770.6641299369340803No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG3760.6623683190642286No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAAG3700.6517986118451186No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3410.6007116936194201No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG3310.5830955149209034No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG3260.574287425571645No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG3240.5707641898319417No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG3240.5707641898319417No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG3050.5372934503047598No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2990.5267237430856498No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2950.5196772716062432No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG2860.5038227107775781No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2500.4404044674629179No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2490.4386428495930662No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG2390.4210266708945496No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG2330.41045696367543955No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2300.40517211006588455No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG2290.40341049219603287No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2280.4016488743261812No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2250.3963640207166262No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2130.3752246062784061No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2100.36993975266885104No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2070.36465489905929604No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2070.36465489905929604No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2040.35937004544974105No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2010.35408519184018605No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1960.3452771024909277No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1900.33470739527181764No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1850.3258993059225593No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG1830.3223760701828559No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1800.3170912165733009No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1660.2924285663953775No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1650.2906669485255258No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1600.28185885917626746No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1600.28185885917626746No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCAG1570.27657400556671247No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1560.2748123876968608No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1490.2624810626078991No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1480.2607194447380474No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1460.2571962089983441No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1400.24662650177923406No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1320.23253355882042068No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCAG1290.22724870521086565No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1280.22548708734101397No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1270.2237254694711623No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1270.2237254694711623No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1270.2237254694711623No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1230.2166789979917556No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1210.2131557622520523No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1200.21139414438220064No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1190.20963252651234895No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1180.2078709086424973No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG1160.20434767290279393No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG1140.2008244371630906No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1130.1990628192932389No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1120.19730120142338725No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1120.19730120142338725No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG1090.19201634781383223No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1090.19201634781383223No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG1030.18144664059472218No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1030.18144664059472218No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1010.17792340485501887No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG990.1744001691153155No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG990.1744001691153155No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG980.17263855124546384No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG970.17087693337561216No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG970.17087693337561216No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG970.17087693337561216No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG950.16735369763590882No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCATCAG920.1620688440263538No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG920.1620688440263538No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG890.15678399041679877No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA880.15502237254694712No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG870.15326075467709546No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG870.15326075467709546No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG860.15149913680724378No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG840.14797590106754044No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG830.14621428319768875No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG810.14269104745798541No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG810.14269104745798541No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCAG790.13916781171828208No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG760.13388295810872705No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCAG750.13212134023887537No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG750.13212134023887537No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG740.1303597223690237No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG740.1303597223690237No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG740.1303597223690237No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG720.12683648662932037No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG710.1250748687594687No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCAG680.11979001514991368No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG670.11802839728006201No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA670.11802839728006201No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG650.11450516154035865No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCAG640.11274354367050698No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG630.11098192580065531No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCAG630.11098192580065531No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG630.11098192580065531No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG630.11098192580065531No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG630.11098192580065531No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG620.10922030793080365No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCAG620.10922030793080365No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG620.10922030793080365No Hit
GACTACTGGGGTATCTAATCCTGTTCGATCCCCACACTTTCGCATCTCAG580.10217383645139697No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCAG580.10217383645139697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTA151.3298306E-5252.9909245
ATCCGGG151.3298306E-5252.9909245
ATCTGGA100.0013374888252.9909245
TTCCGGA5600.0250.73206245
ATCCGGA12350.0248.89389245
TACTACT350.0245.182541
TACTACC151.507301E-5245.182541
GACTACT22900.0245.182541
TACTGGG12700.0244.966544
CTACTGG12700.0244.966543
ACTACAA6350.0244.966542
CTACAGG8200.0244.966523
GGGTCTC800.0244.966529
GGGTTTC151.5126187E-5244.966529
ACTCGGG5250.0244.966525
GTGTATC301.6370905E-11244.966529
TGGGGTC201.5498517E-7244.966527
CAGGGTC100.0014731195244.966527
TACAAGG6400.0244.966524
CAGGGGT8100.0244.966526
Produced by FastQC (version 0.11.5)