FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005308074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35616977
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3235860.9085161831673698Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2797880.7855467351987789No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT706330.1983127315942619Clontech SMART CDS Primer II A (100% over 24bp)
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC383220.10759475741020919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)