FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005308095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104231261
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA2358900.22631406138317756No Hit
CCACCTACTAAGAACGGCCATGCACCAC1886420.18098409075181388No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA1506450.14452957640030856No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG1223900.11742158621682605No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC1166140.11188006254668646No Hit
GATCGGAAGAGCACACGTCTGAACTCCA1134350.10883011383696105Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTTTAAGTTTCAGCTTTGCAACCATAC1121240.10757233379340964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)