FastQCFastQC Report
Thu 12 May 2022
EGAF00005308113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113159440
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA5630030.49753074069648984No Hit
CCACCTACTAAGAACGGCCATGCACCAC2903350.2565716125848626No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG2615740.23115526199139905No Hit
CCACCAACTAAGAACGGCCATGCACCAC1824270.16121235665358544No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA1812280.1601527897274854No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC1780850.15737529277274614No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA1666220.14724533808226692No Hit
GATCGGAAGAGCACACGTCTGAACTCCA1345510.11890391115403187Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTCCTAATCATGGCCTCAGTTCCGAA1131880.1000252387251121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)