FastQCFastQC Report
Thu 12 May 2022
EGAF00005308117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130730953
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA5978240.457293384834424No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA2947170.22543781196179302No Hit
CCACCTACTAAGAACGGCCATGCACCAC2636360.20166302925979587No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG2456950.18793942395570237No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA2102600.1608341369621929No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC2043290.15629733839697474No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT1673010.12797351825317146No Hit
CCACCAACTAAGAACGGCCATGCACCAC1640170.1254614888334823No Hit
CACCTTGTTACGACTTTTACTTCCTCTA1418610.10851370447823476No Hit
GATCGGAAGAGCACACGTCTGAACTCCA1403060.1073242386598375Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTCCTAATCATGGCCTCAGTTCCGAA1352730.10347434704312145No Hit
GGGCATCACAGACCTGTTATTGCTCAAT1321480.10108394145952565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)