FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005308177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111015239
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA4603350.4146592883522955No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA2516110.22664546080921377No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG2465970.22212896375424637No Hit
CCACCTACTAAGAACGGCCATGCACCAC2439820.21977343128541119No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC1785350.16082026360363014No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA1670440.15046943239927627No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT1419580.1278725346886836No Hit
CCACCAACTAAGAACGGCCATGCACCAC1391950.12538368718910742No Hit
GATCGGAAGAGCACACGTCTGAACTCCA1326950.11952863516332204Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)