FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005308179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115190918
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA4811860.41772911298441084No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA2679940.2326520220977838No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG2636860.22891214392440207No Hit
CCACCTACTAAGAACGGCCATGCACCAC2554210.22173709910012174No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC1875830.162845303481304No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA1756520.15248771608886735No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT1483810.12881310660272713No Hit
CCACCAACTAAGAACGGCCATGCACCAC1445070.12544999424346978No Hit
GATCGGAAGAGCACACGTCTGAACTCCA1348200.11704047709733506Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)