FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005308281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125494440
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA5727920.4564281891691775No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA2815850.22438045860836545No Hit
CCACCTACTAAGAACGGCCATGCACCAC2537820.20222569222987088No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG2321350.1849763224569949No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC1982390.1579663609001323No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA1959750.15616229691132133No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT1611000.12837222111194727No Hit
CCACCAACTAAGAACGGCCATGCACCAC1581670.12603506577661927No Hit
CACCTTGTTACGACTTTTACTTCCTCTA1341950.106933024283785No Hit
GATCGGAAGAGCACACGTCTGAACTCCA1315350.10481340846654243Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTCCTAATCATGGCCTCAGTTCCGAA1274720.10157581483291211No Hit
GGGCATCACAGACCTGTTATTGCTCAAT1272380.1013893523888389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)