FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005308283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124997790
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA5703330.45627446693257534No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA2797290.2237871565569279No Hit
CCACCTACTAAGAACGGCCATGCACCAC2516210.20130035899034696No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG2294630.1835736455820539No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA1958240.1566619697836258No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC1958180.15665716969876028No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT1591970.12735985172217845No Hit
CCACCAACTAAGAACGGCCATGCACCAC1577520.12620383128373708No Hit
GATCGGAAGAGCACACGTCTGAACTCCA1355880.10847231779057853Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACCTTGTTACGACTTTTACTTCCTCTA1329900.10639388104381686No Hit
GGGCATCACAGACCTGTTATTGCTCAAT1269900.10159379617831643No Hit
CCCTCCTAATCATGGCCTCAGTTCCGAA1263750.10110178747960263No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)