FastQCFastQC Report
Thu 12 May 2022
EGAF00005308289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130016966
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA5961640.4585278508960131No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA2921960.224736823961882No Hit
CCACCTACTAAGAACGGCCATGCACCAC2635740.20272277388783244No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG2470490.19001289416336634No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA2094010.16105667317294575No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC2073040.1594438067413448No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT1664330.12800867849815847No Hit
CCACCAACTAAGAACGGCCATGCACCAC1644650.12649502988709949No Hit
CACCTTGTTACGACTTTTACTTCCTCTA1409640.10841969654944879No Hit
GATCGGAAGAGCACACGTCTGAACTCCA1381430.10624997971418593Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTCCTAATCATGGCCTCAGTTCCGAA1323740.1018128664839018No Hit
GGGCATCACAGACCTGTTATTGCTCAAT1316830.10128139738316921No Hit
AGGTCCGTCTTGCGCCGGTCCAAGAATT1307680.1005776430746738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)