FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005308346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53616134
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22699434.2336939101204125Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6274421.1702484927391446Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3609040.6731257423371854No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1861840.34725368300519394Clontech SMART CDS Primer II A (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1536170.28651263815477634Clontech SMART CDS Primer II A (100% over 21bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1226730.22879866720715075Clontech SMART CDS Primer II A (100% over 22bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1094670.20416802151382268Clontech SMART CDS Primer II A (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)