FastQCFastQC Report
Thu 12 May 2022
EGAF00005308573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115889890
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA4826800.416498798989282No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA2709000.23375636994736987No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG2629080.22686016873430462No Hit
CCACCTACTAAGAACGGCCATGCACCAC2555880.22054382828389948No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC1855530.16011146442541277No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA1764860.15228765856969922No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT1486900.12830282261895323No Hit
CCACCAACTAAGAACGGCCATGCACCAC1447630.124914261287158No Hit
GATCGGAAGAGCACACGTCTGAACTCCA1376500.11877653866096516Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)