FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005308589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120456390
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA4144670.3440805423439969No Hit
CCACCTACTAAGAACGGCCATGCACCAC2844890.23617593055876904No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA2116590.1757142149121354No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG2101090.1744274421639234No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC1610730.13371893346629432No Hit
CCACCAACTAAGAACGGCCATGCACCAC1601260.13293275682593508No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA1502720.12475220285117294No Hit
GATCGGAAGAGCACACGTCTGAACTCCA1365560.11336550929344637Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGCCAGAGTCTCGTTCGTTATCGGAAT1210190.10046706530056229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)