FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005308593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120937775
Sequences flagged as poor quality0
Sequence length28
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA4159730.34395622046130747No Hit
CCACCTACTAAGAACGGCCATGCACCAC2862880.23672338936283557No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA2138320.1768115876118938No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG2111180.17456745834789833No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC1626780.1345138026559526No Hit
CCACCAACTAAGAACGGCCATGCACCAC1600650.13235318741394078No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA1505170.12445821828622199No Hit
GATCGGAAGAGCACACGTCTGAACTCCA1350370.11165824739209895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGCCAGAGTCTCGTTCGTTATCGGAAT1229690.10167956207231363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)